rabbit anti‐α 2a –ar  (Proteintech)

Journal:

Article Title: Potentiation of Spinal ? 2 -Adrenoceptor Analgesia in Rats Deficient in TRPV1- Expressing Afferent Neurons

doi: 10.1016/j.neuropharm.2007.03.009

Figure Lengend Snippet: Confocal images showing the effect of RTX on α2A-AR- and TRPV1-immmunoreactivity in the spinal cord dorsal horn. Representative confocal images showing α2A-AR- and TRPV1-immunoreactivities in the spinal dorsal horn of one vehicle- and one RTX-treated rat. All images are single confocal optical sections. Scale bar, 100 μm. Inset: high-magnification images (scale bar = 10 μm) showing co-localization of α2A-AR- and TRPV1-immunoreactivity in the lamina I and II. Co-localization is indicated in yellow when two images are digitally merged.

Article Snippet: Sections intended for the labeling of TRPV1 and α 2A -AR were incubated in a mixture of primary antibodies (guinea pig anti-TRPV1, dilution: 1:1000; and rabbit anti-α 2A -AR, dilution 1:100, Neuromics, Minneapolis, MN) for 2 hr at room temperature and overnight at 4°C.

Techniques:

Journal:

Article Title: Potentiation of Spinal ? 2 -Adrenoceptor Analgesia in Rats Deficient in TRPV1- Expressing Afferent Neurons

doi: 10.1016/j.neuropharm.2007.03.009

Figure Lengend Snippet: Double immunofluorescence labeling showing α2C-AR- and TRPV1-immmunoreactivity in the spinal cord dorsal horn of one vehicle-treated and one RTX-treated rat. Representative confocal images showing α2C-AR- and TRPV1-immunoreactivities in the spinal dorsal horn of one vehicle- and one RTX-treated rat. All images are single confocal optical sections. Scale bar, 100 μm. Inset: high-magnification images (scale bar = 10 μm) showing the distribution of α2C-AR- and TRPV1-immunoreactivity in the lamina I and II.

Article Snippet: Sections intended for the labeling of TRPV1 and α 2A -AR were incubated in a mixture of primary antibodies (guinea pig anti-TRPV1, dilution: 1:1000; and rabbit anti-α 2A -AR, dilution 1:100, Neuromics, Minneapolis, MN) for 2 hr at room temperature and overnight at 4°C.

Techniques: Immunofluorescence, Labeling